Guy G. Poirier

Research Interests

• Poly(ADP-ribose) polymerases (PARPs) and poly(ADP-ribosylation)
• Ovarian cancer
• PARPs inhibitors
• DNA damage response and repair mechanism studies

Current Research Projects

• Study of the role of poly(ADP-ribosylation) in DNA damage response mechanisms
• Analysis of the role of poly(ADP-ribose) binding proteins in DNA repair by homologous recombination
• Identification of proteins involved in the mechanisms of sensitivity and resistance to PARP inhibitors
• Study of the molecular mechanisms involved in the concept of synthetic lethality with PARP inhibitors

Tools and Expertises

• Mass spectrometry and affinity purification of poly(ADP-ribose) binding protein complexes
• Confocal microscopy analysis of the localization dynamics of signaling and repair factors to DNA lesions generated by micro-irradiation
• Synthesis, purification and characterization of poly(ADP-ribose)
• Identification and characterization of poly(ADP-ribose) binding proteins
• High throughput cell microscope (Sartorius Incucyte SX5)
• X-ray irradiation of cells